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Plant Pathology

Department of Plant Pathology


McSpadden Gardener publications


Kim, Hyun Jung); Lee, Jin Hee; Kang, Beom Ryong; Rong, Xiaoqing; McSpadden Gardener, Brian B; Ji, Hyung Jin; Park, Chang-Seuk; Kim, Kim, Young Cheol. Draft Genome Sequence of Pantoea ananatis B1-9, a Nonpathogenic Plant Growth-Promoting Bacterium.  Journal of Bacteriology 194(3): 729-729.

Kim, Hyun Jung; Park, Ju Yeon; Han, Song Hee; Lee, Jin Hee; Rong, Xiaoqing; McSpadden Gardener, Brian B.; Park, Seur Kee; Kim, Young Cheol. 2012. Genome Sequence of the Biocontrol Bacterium Chromobacterium sp Strain C-61. Journal of Bacteriology 193(23): 6803-6804.

Rong, X., Baysal Gurel, F., Meulia, T. and B.B. McSpadden Gardener. 2012. Draft Genome Sequences of the Pseudomonas fluorescens Biocontrol Strains Wayne1R and Wood1R.  Journal of Bacteriology 194: 724-725.

Rong, X., Baysal Gurel, F., Meulia, T., and B.B. McSpadden Gardener. 2012. Draft Genome Sequences of the Biocontrol Bacterium Mitsuaria sp. Strain H24L5A.  Journal of Bacteriology 194: 734-735.


Cho SM, Park JY, Han SH, Anderson AJ, Yang KY, Gardener BBM, and Kim YC. 2011. Identification and transcriptional analysis of priming genes in Arabidopsis thaliana induced by root colonization with Pseudomonas chlororaphis O6. The Plant Pathology Journal 23:272-279.

Kim, Y.C., Leveau, J., McSpadden Gardener, B.B. Pierson, E.A., Pierson, L.S. III,  and C.-M. Ryu. 2011. The multifactorial basis for plant health promotion by plant-associated bacteria. Appl Environ. Microbiol. Appl Environ. Microbiol. 77:1548-1555. > pdf file

Rodrguez GR, Munos S, Anderson C, Sim S-C, Michel A, Causse M, McSpadden Gardener B., Francis F, and E van der Knaap. 2011. Distribution of SUN, OVATE, LC, and FAS in the Tomato Germplasm and the Relationship to Fruit Shape Diversity Plant Physiol. 156: 275-285.

Westphal, A. Williams, M.L. Baysal-Gurel, F., LeJeune, J.T. and B.B. McSpadden Gardener. 2011. General suppression of Escherichia coli O157:H7 indicated in sand-based dairy livestock bedding. Appl. Environ. Microbiol. 77:2113-2121. > pdf file


McSpadden Gardener, B. 2010. Biocontrol of Plant Pathogens and Plant Growth Promotion by Bacillus. Chapter 6 in Recent Developments in Disease Management, Vol.1, U. Gisi, I. Chet, M. Lodovica Gullino eds. Springer; Amsterdam.  377 pp.

Park, S., Cheng, Z., Yang, H., Morris, E.E., Sutherland, M., McSpadden Gardener, B.B., and Grewal, P. 2010. Differences in soil chemical properties with distance from roads and age of development in urban areas. Urban Ecosystems 13:483-497.

Park, S., Cheng, Z.,McSpadden Gardener, B.B., and Grewal, P. 2010. Are nematodes effective bioindicators of soil conditions and processes along distance from roads and age of development in urban areas? Journal of Environmental Indicators 5:28-47.

Park, S., Cao, C. and McSpadden Gardener, B.B. 2010. Inoculants and soil amendments for organic growers. OSU Fact Sheet.  7 pp.

Cao, C., Park, S. and McSpadden Gardener, B.B. 2010. Biopesticide controls for plant diseases: Resources and products for organic farmers in Ohio. OSU Fact Sheet.  10 pp.

McGrath, M., Vallad, G., and McSpadden Gardener. B. 2010. Biopesticides for Plant Disease Management in Organic Farming. eXtension. Published Aug 23, 2010 online at:


Benitez, Maria-Soledad and McSpadden Gardener, B. B.  2009.   Linking sequence to function in soil bacteria: Sequence-directed isolation of bovel bacteria contributing to soilborne plant disease suppression.  Applied and Environmental Microbiology, Feb. 2009, pp. 915-924.

Raudales, R.E., Stone, E., and McSpadden Gardener, B. B. 2009, Seed treatment with 2,4-diacetylphloroglucinol-producing Pseudomonads improves crop health in low-pH soils by altering patterns of nutrient uptake. Phytopathology 99(5): 506-511.

Timper, P. Kone, D., Yin, J., Pingsheng, J., and McSpadden Gardener, B.B. 2009. Evaluation of an antibiotic producing strain of Pseudomonas fluorescens for suppression of plant-parasitic nematodes. J. Nematology 41:234-240


Baysal, F., Benitez, M.-S., Kleinhenz, M.D., Miller, S.A., and McSpadden Gardener, B.B. 2008. Effects of farm organic transition on soilborn disease suppression. Phtyopathology 98: 562-570. PDF

Hoitink, H.A.J., McSpadden Gardener, B.B., and Miller, S.A. 2008. Current knowledge on disease suppressive properties of composts. Pages 19-25 ini Compost and digestate: sustainability, benefits, impacts of the environment and for plant production. Proceedings of the international congress CODIS 2008 Feb 27-29, 2008. Solothum, Switzerland. Research Institute for Organic Agriculture: Ackerstrasse, Switzerland. PDF

Raudales, R.E. and McSpadden Gardener, B.B. 2008. Microbial biopesticides for control of plant diseases in organic agriculture. OSU Fact Sheet. 5 pp.

Wallis, C., Ayles, E., Chorbadjian, R., McSpadden Gardener, B., Hansen, R., Cippolini, D., Herms, D.A., and P. Bonello 2008. Systemic induction of phloem secondary metabolism and induction of resistance to a canker pathogen in Austrian pine. New Phytologist 177:767-778. PDF


Borneman, J., Becker, J.O., Bent, E., Lanoil, B., McSpadden Gardener, B., Olatinwo, R., Presley, L., Scupham, A.J., Valinsky, L., and Yin, B. 2007. Identifying microorganisms involved in specific in situ functions: Experimental design considerations for rRNA gene-based population studies and sequence-selective PCR assays. Pages 748-757. in Manual of Environmental Microbiology, 3rd ed. ASM Pres: Washington, D.C.

Benitez, M.-S., Baysal Tustas, F., Rotenberg, D., Kleinhenz, M.D., Miller, S.A., and McSpadden Gardener, B.B. 2007. Multiple statistical approaches of community fingerprint data reveal bacterial populations associated with general disease suppression arising from the application of different organic field management strategies. Soil Biology and Biochemistry 39:2289-2301. PDF

Lewis Ivey, M.L., McSpadden Gardener, B.B., Opina, N., and Miller, S.A. 2007. Diversity and geographic distribution of Ralstonia solanacearum strains isolated from eggplant in the Luzon Region of the Philippines. Phytopathology 97:1467-1475.

McSpadden Gardener, B. 2007. Diversity and ecology of biocontrol Pseudomonas in agricultural systems. Phytopathology 97:221-226. PDF

Mullen, R.W., McSpadden Gardner, B., Raudales, R., and Dygert, C.E. 2007. Swine manure applications for soybean production - environmental and pathological implications. North Central Extension-Industry Soil Fertility Conference Proceedings Vol. 23. Pgs 24-29.

Rotenberg, D. Joshi, R. Benitez, M.S., Gutierrez Chapin, L., Camp, A., Zumpetta, C., Osborne, A., Dick, W.A., and McSpadden Gardener, B.B. 2007. Complex effects of farm management on rhizosphere colonization by native populations of phlD-containing Pseudomonas spp. and the relative contribution of those bacteria to crop stand and productivity. Phytopathology 97:756-766. PDF


Pal, K.K. and B. McSpadden Gardener. 2006. Biological control of plant pathogens. The Plant Health Instructor. DOI:10.1094/PHI-A-2006-1117-02. 25 pp. Online:

Joshi, R., and McSpadden Gardener, B. 2006. Identification of genes associated with pathogen inhibition in different strains B. subtilis. Phytopathology 96:145-154. PDF

Gutierrez Chapin, L., Want, Y., Lutton, E., and McSpadden Gardener, B. 2006. Distribution and fungicide sensitivity of tomato fruit rot pathogens in Ohio. Plant Disease 90:397-403. PDF

McSpadden, Gardener, B. 2006. Farm-based biocontrol seed treatments for improving soybean yields. Available on Organic Ag info ( and OFFER web-site (

McSpadden Gardener, B. 2006. Statistical analyses of microbiological and environmental data. Chapter 22, pp. 555-585 in Modern Soil Microbiology, 2nd ed. J.D. van Elsas, J. Jannson eds. CRC Press: Boca Raton, FL PDF

McSpadden Gardener, B., C. Kroon van Diest, and J. Beuerlein 2006. Evaluation of biological seed treatments containing phlD+ strains of Pseudomonas fluorescens on soybeans grown in Ohio, 2005. Biological and Cultural Tests 21:FC045

McSpadden Gardener, B., M. S. Benitez, A. Camp, C. Zumpetta 2006. Evaluation of a seed treatment containing a phlD+ strain of Pseudomonas fluorescens on organic soybeans, 2005. Biological and Cultural Tests 21:FC046


McSpadden Gardener, B., Gutierrez, L., Joshi, R., Edema, R., and Lutton, E. 2005. Distribution and biocontrol potential of phlD+ pseudomonads in corn and soybean fields. Phytopathology 95:715-724. PDF

Connor, C.J., Luo, H., McSpadden Gardener, B., and Wang, H. 2005. Development of a real-time PCR-based system targeting the 16S rRNA gene sequence for rapid detection of Alicyclobacillus spp. in juice products. Int. J. Food Microbiol. 99:229-235. PDF


McSpadden Gardener, B. 2004. Ecology of Bacillus and Paenibacillus spp. in agricultural systems. Phytopathology 94:1252-1258. PDF

Blouin Bankhead, S., Landa,B., Lutton, E., Weller D., McSpadden Gardener, B. 2004. Minimal changes in rhizobacterial population structure following root colonization by wild type and transgenic biocontrol strains. FEMS Microbiol. Ecol. 49:307-318. PDF

McSpadden Gardener, B. and J. Beuerlein. 2004. Evaluation of rhizobial inoculants with and without Bacillus subtilis MBI600 on soybeans in Ohio. B&C Tests DOI:10.1094/BC19:FC035

McSpadden Gardener, B., A. Replogle, A. Osborne, and E. Lutton. 2004. Evaluation of seed treatments containing Bacillus biocontrol strains on soybeans. B&C Tests DOI:10.1094/BC 19:FC038

Gutierrez, L., McSpadden Gardener, B. 2004. Tomato fruit rot pathogens resistant to fungicides. The Vegetable Growers News 38(3): 36-37.


Weller, D., Raaijmakers, J., McSpadden Gardener, B., Thomashow, L. 2002. Microbial populations responsible for specific soil suppressiveness to plant pathogens. Ann. Rev. Phytopathol. 40:309-348. PDF

McSpadden Gardener, B., Fravel, D. 2002. Biological control of plant pathogens: Research, commercialization, and application in the USA. Online. Plant Health Progress doi:10.1094/PHP-2002-0510-01-RV.

Mavrodi, D., Mavrodi, O., McSpadden Gardener, B., Landa, B., Weller, D., Thomashow, L. 2002. Identification of differences in genome content among phlD-positive Pseudomonas fluorescens using PCR-based subtractive hybridization. Appl. Environ. Microbiol. 68:5170-5176.

McSpadden Gardener, B., Miller, S., Kleinhenz, M., Doohan, D., Grewal, P., Stinner, D. 2002. Dried and composted dairy manure in vegetable cropping systems: Effects on soil and plant health. pgs. 581-591 In Proceedings of the 2002 International Compost Symposium: Composting and Compost Utilization. The JG Press, Inc: Emmaus, PA. ( ).

Landa, B., Mavrodi, O., Raaijmakers, J. McSpadden Gardener, B., Thomashow, L., Weller, D. 2002. Certain genotypes of 2,4-diacetylphloroglucinol-producing Pseudomonas fluorescens strains preferentially colonize the rhizosphere of pea. Appl. Environ. Microbiol. 68:3226-3237.

Landa, B., de Werd, H., McSpadden Gardener, B., and Weller, D. 2002. Comparison of three methods for monitoring populations of different genotypes of 2,4-diacetylphloroglucinol-producing Pseudomonas fluorescens in the rhizosphere. Phytopathology 92:129-137.


McSpadden Gardener, B., Weller, D. 2001. Changes in populations of rhizosphere bacteria associated with take-all disease of wheat. Appl. and Environ. Microbiol. 67:4414-4425. PDF

McSpadden Gardener, B., Mavrodi, D., Thomashow, L., Weller, D. 2001. A rapid polymerase chain reaction-based assay characterizing rhizosphere populations of 2,4-DAPG-producing-bacteria. Phytopathology 91: 44-54. PDF

Mavrodi, O., McSpadden Gardener, B., Mavrodi, D., Bonsall, R., Weller, D., Thomashow, L. 2001. Genetic diversity of phlD from 2,4-DAPG-producing fluorescent Pseudomonas spp. Phytopathology 91:35-43.


McSpadden Gardener, B., Schroeder, K., Kalloger, S., Raaijmakers, J., Thomashow, L., Weller, D. 2000. Genotypic and phenotypic diversity of phlD-containing Pseudomonas spp. isolated from the rhizosphere of wheat. Appl. Environ. Microbiol. 66:1939-1946. PDF

McSpadden Gardener, B., de Bruijn, F. 1998. Detection and isolation of novel rhizopine- catabolizing bacteria from the environment. Appl. Environ. Microbiol. 64:4944-4949. PDF

Van Elsas, J., McSpadden Gardener B., Wolters, A., Smit, E. 1998. Isolation, characterization, and transfer of cryptic gene-mobilizing plasmids in the wheat rhizosphere. Appl. Environ. Microbiol. 64:880-889.

Zhang L, McSpadden B, Pakrasi H, Whitmarsh J. 1992. Copper-mediated regulation of cytochrome b553 and plastocyanin in Synochocystis PCC 6803. J. Biol. Chem. 267: 19054-19059.