Plant innate immunity/disease resistance; especially as applied to soybean. (Photo credit: Ken Chamberlain)
A) Soybean defense-related genes induced by a PAMP (Pathogen Associated Molecular Pattern), the Phytophthora cell wall glucan elicitor: Up-regulations of flavonoid/isoflavonoid metabolic genes, Pathogenesis Related (PR) protein genes as well as other novel genes revealed by microarray studies with this elicitor.
B) Genes induced during priming of soybean defense responses by cell death causing chemicals: Herbicide lactofen induces cell death marker genes as well as metabolic and PR genes. Natural chemicals fumonisin and juglone induce novel genes as revealed with Next generation mRNA sequencing.
C) Functional studies of soybean defense-related genes using Agrobacterium rhizogenes mediated RNAi gene silencing and assignment of their roles in general (PAMP-triggered Immunity; PTI) vs race-specific resistance (effector-triggered Immunity; ETI)
D) Initiation of PAMP recognition pathway at the host cell surface: Probing membrane pattern recognition receptors (PRR’s) in soybean and its evolutionary context.
BS Agricultural Chemistry, National Taiwan University
PhD Biochemistry, Purdue University
Post-Doctoral Pharmacology, University of Wisconsin
NIH Trainee Microbiology and Immunology, Washington University
1981-1985 Research Associate, Genetics and Pharmacology, Washington University
1986-1990 Research Associate, Biotechnology Center and Plant Pathology, Ohio State University
1991-1996 Senior Research Associate, Plant Pathology/OARDC, Ohio State University
1997-2005 Research Scientist, Plant Pathology, Ohio State University
2005- current Senior Programatic Research Scientist, Plant Pathology, Ohio State University
Graham MY, Subramanian S, Yu O, Graham TL. 2010. Isoflavonoids participate in hypersensitive cell death during race-specific soybean-Phytophthora interactions. In: Biology of Plant-Microbe Interactions, Vol. 7., Antoun H, Avis T, Brisson L, Prévost D and Trepanier M. APS Press.
Graham TL, Graham MY, Yu O. 2008. Genomics of Soybean Secondary Product Metabolism. In: Genetics and Genomics of Soybean, Stacey G, ed. Springer Verlag, NY, pp. 211-242.
Graham MY, Sinden MR, Huge R, Subramaniam S, Yu O, St. Martin S, Graham TL. 2008 “Silencing of defense-related genes reveals different mechanisms leading to race-specific resistance to Phytophthora in soybean” Phytopathology 98 (6): S61
Graham TL, Graham MY, Subramanian S, Yu O. 2007. RNAi silencing of genes for elicitation or biosynthesis of 5-deoxyisoflavonoids suppresses race specific resistance and hypersensitive cell death in Phytophthora sojae infected tissues. Plant Physiol. 144: 728-740.
Subramanian S, Graham MY, Yu O, Graham TL. 2005 RNA Interference of soybean isoflavone synthase genes leads to silencing in tissue distal to the transformation site and to enhanced susceptibility to Phytophthora sojae. Plant Physiol. 137:1345-1353
Graham MY. 2005 The diphenylether herbicide Lactofen induces cell death and expression of defense-related genes in soybean. Plant Physiol. 139:1784-1794
Graham MY, Weidner J, Wheeler K, Pelow MJ, Graham TL. 2003 Induced expression of pathogenesis-related protein genes in soybean by wounding and the Phytophthora sojae cell wall glucan elicitor. Physiol. Molec. Plant Pathol. 63: 141-149
Manuscript in preparation
Graham MY, Kelly MR, Farmer A, Kim R, Graham TL. Natural chemicals fumonisin and Juglone induce novel metabolites and genes for priming soybean defense activation.
Campus-wide program: Plant Molecular Biology and Biotechnology
State-wide program: Ohio Plant Biotechnology Consortium
National Societies: American Phytopathological Society (APS), American Society of Plant Biologists (ASPB)
International Society: International Society of Molecular Plant-Microbe Interactions (IS-MPMI)