PLNTPTH 3002
The lab portion of general plant pathology. Lab work will include experiments involving fungi, bacteria, viruses, nematodes and parasitic higher plants. Video-linked to Wooster.
Au Sem. Prereq: Biology 1101 (101), 1113 (113), 1115H (115H), or Entmlgy 1101 (Entomol 101). Concur: 3001 (401). Not open to students with credit for 6001 or 401.
Notes:
Sample list of topics
- Disease Walks to help with terminology: Signs, Symptoms, Disease, Injury, Pathogen, Abiotic, Biotic
- Abiotic Stresses: Light Stress (Plant soybeans and corn, place in greenhouse)
- Abiotic Stresses: Nutrient Availability (plant soybeans, do pH test, fill out soil sample forms)
- Microscopes and Their Use in Diseases Diagnostics: Use hand lenses and microscopes to observe samples – observe both healthy (asymptomatic) and unhealthy specimens
- Mechanical Transmission of TMV; Discuss Use of green fluorescent protein (GFP) and establish TMV-GFP mutant demonstration; Discuss general safeguards needed when working with GFP modified organism; ELISA; Use of a Lateral Flow Immunostrip ELISA.
- Aseptic Technique and Preparation of Microbiological Media
- Bacterial streaming
- Isolation of a bacterial plant pathogen; Discuss how plant pathologists (and microbiologists) characterize plant pathogenic bacteria
- Bacterial Soft Rot of Vegetables
- Field & Woodland Fungal Foray
- Oomycetes: Chemical Management of Pythium Foliar Blight of Perennial Ryegrass and observation of Pythium aphanidermatum; zoospore release exercise
- Isolation of Fungal-like or Fungal Pathogens; hyphal tip transfer isolates
- Fungicide resistance in S. homoeocarpa
- Keying Out of Powdery Mildew Fungi
- Apple Scab
- Dutch Elm Disease
- Basidiomycetes: The Smuts: Corn smut; Stinking smut of wheat; Loose smut of wheat
- Basidiomycetes: The Rusts: Black stem rust of wheat and Cedar-apple rust
- Nematodes Extraction and observation of soil-borne plant pathogenic nematodes
Course Level:
Undergraduate
Unit(s):
2.0
Semesters Offered:
Autumn 2023
Current Instructors:
Instruction Modes:
In Person
Campuses:
Columbus
Program area(s):
Course Subject: