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Plant Pathology

Department of Plant Pathology

CFAES

General Plant Pathology Lab

PLNTPTH 3002
The lab portion of general plant pathology. Lab work will include experiments involving fungi, bacteria, viruses, nematodes and parasitic higher plants. Video-linked to Wooster. Au Sem. Prereq: Biology 1101 (101), 1113 (113), 1115H (115H), or Entmlgy 1101 (Entomol 101). Concur: 3001 (401). Not open to students with credit for 6001 or 401.
Notes: 

Sample list of topics

  • Disease Walks to help with terminology: Signs, Symptoms, Disease, Injury, Pathogen, Abiotic, Biotic
  • Abiotic Stresses: Light Stress (Plant soybeans and corn, place in greenhouse)
  • Abiotic Stresses: Nutrient Availability (plant soybeans, do pH test, fill out soil sample forms)
  • Microscopes and Their Use in Diseases Diagnostics: Use hand lenses and microscopes to observe samples – observe both healthy (asymptomatic) and unhealthy specimens
  • Mechanical Transmission of TMV; Discuss Use of green fluorescent protein (GFP) and establish TMV-GFP mutant demonstration; Discuss general safeguards needed when working with GFP modified organism; ELISA; Use of a Lateral Flow Immunostrip ELISA.
  • Aseptic Technique and Preparation of Microbiological Media
  • Bacterial streaming
  • Isolation of a bacterial plant pathogen; Discuss how plant pathologists (and microbiologists) characterize plant pathogenic bacteria
  • Bacterial Soft Rot of Vegetables
  • Field & Woodland Fungal Foray
  • Oomycetes: Chemical Management of Pythium Foliar Blight of Perennial Ryegrass and observation of Pythium aphanidermatum; zoospore release exercise
  • Isolation of Fungal-like or Fungal Pathogens; hyphal tip transfer isolates
  • Fungicide resistance in S. homoeocarpa
  • Keying Out of Powdery Mildew Fungi
  • Apple Scab
  • Dutch Elm Disease
  • Basidiomycetes: The Smuts: Corn smut; Stinking smut of wheat; Loose smut of wheat
  • Basidiomycetes: The Rusts: Black stem rust of wheat and Cedar-apple rust
  • Nematodes Extraction and observation of soil-borne plant pathogenic nematodes
Course Level: 
Undergraduate
Unit(s): 
2.0
Semesters Offered: 
Autumn 2021
Current Instructors: 
Instruction Modes: 
In Person
Campuses: 
Columbus
Course Subject: