General Plant Pathology Lab

PLNTPTH 3002
The lab portion of general plant pathology. Lab work will include experiments involving fungi, bacteria, viruses, nematodes and parasitic higher plants. Video-linked to Wooster. Au Sem. Prereq: Biology 1101 (101), 1113 (113), 1115H (115H), or Entmlgy 1101 (Entomol 101). Concur: 3001 (401). Not open to students with credit for 6001 or 401.
Notes: 

Sample list of topics

  • Disease Walks to help with terminology: Signs, Symptoms, Disease, Injury, Pathogen, Abiotic, Biotic
  • Abiotic Stresses: Light Stress (Plant soybeans and corn, place in greenhouse)
  • Abiotic Stresses: Nutrient Availability (plant soybeans, do pH test, fill out soil sample forms)
  • Microscopes and Their Use in Diseases Diagnostics: Use hand lenses and microscopes to observe samples – observe both healthy (asymptomatic) and unhealthy specimens
  • Mechanical Transmission of TMV; Discuss Use of green fluorescent protein (GFP) and establish TMV-GFP mutant demonstration; Discuss general safeguards needed when working with GFP modified organism; ELISA; Use of a Lateral Flow Immunostrip ELISA.
  • Aseptic Technique and Preparation of Microbiological Media
  • Bacterial streaming
  • Isolation of a bacterial plant pathogen; Discuss how plant pathologists (and microbiologists) characterize plant pathogenic bacteria
  • Bacterial Soft Rot of Vegetables
  • Field & Woodland Fungal Foray
  • Oomycetes: Chemical Management of Pythium Foliar Blight of Perennial Ryegrass and observation of Pythium aphanidermatum; zoospore release exercise
  • Isolation of Fungal-like or Fungal Pathogens; hyphal tip transfer isolates
  • Fungicide resistance in S. homoeocarpa
  • Keying Out of Powdery Mildew Fungi
  • Apple Scab
  • Dutch Elm Disease
  • Basidiomycetes: The Smuts: Corn smut; Stinking smut of wheat; Loose smut of wheat
  • Basidiomycetes: The Rusts: Black stem rust of wheat and Cedar-apple rust
  • Nematodes Extraction and observation of soil-borne plant pathogenic nematodes
Course Level: 
Undergraduate
Unit(s): 
2.0
Semesters Offered: 
Autumn 2019
Instruction Modes: 
In Person
Campuses: 
Columbus
Wooster
Course Subject: